Copyright 2015. Time Bioscience. All Rights Reserved.

Recombinant Wnt Proteins

The working concentrations of mouse Wnt3a for organoid culture

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Wnt Ligand Stabilizer:

           We proudly announce that we provide Wnt ligand stabilizer. the Stabilizer helps to maintain Wnt ligand activity for more than 30 hours in serum-free culture conditions.

Wnt Ligands:

          Our Wnt ligands are intact, potent, and fully functional.

          Potent: The Wnt3a has been proved three times more potent than any one else; 30 to 100 ng/mL for organoid formation;

          Intact: These Wnt ligands are purified using a Wnt ligands-binding molecule-conjugated sepharose beads and size exclusion chromatography. The affinity chromatography yields a single pool with strong Wnt activity rather than two pools;

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          Fully functional: The size exclusion chromatography removes dimers and cleaved Wnt proteins. Compared to two Wnt peaks from affinity chromatography, the Wnt3a is fully functional.

​Human Wnt3a:

          We express and purify human Wnt3a from a special human cell line. Our human Wnt3a is as potent as the mouse one.

​Publications:​

Lijian Hui Lab at Chinese Academy of Sciences, China - In Vitro Expansion of Primary Human Hepatocytes
with Efficient Liver Repopulation Capacity. Cell Stem Cell 23, 1–14, December 6, 2018

Ethan Lee lab at Vanderbilt -APC Inhibits Ligand-Independent Wnt Signaling by the Clathrin Endocytic Pathway. Developmental Cell 44, 566–581 (2018)

Leslie S. Gewin Lab at Vanderbilt -Blocking TGF-b and b-Catenin Epithelial Crosstalk Exacerbates CKD. Journal of the American Society of Nephrology 28; 3490-3503 (2017)

Lawrence Lum's Lab at UT Southwestern Medical Center - PNAS Early Edition, J. Moon

Organizations who have proved our Wnt ligands

• Eli Lilly in Spain
  
• Sun Bioscience in Switzerland
  
• STEMCELL Technologies
  
• StemSynergy Therapeutics
• BioLegend
  
• Creative BioMart
• ​Janssen  ​
• Kallyope
• Ethan Lee's Lab at Vanderbilt University School of Medicine
• Xi He's Lab at Harvard Medical School
• Dan Wu's Lab at Yale University School of Medicine
• ​Anthony Brown's Lab at Weill Cornell Medicine
  
• Derk ten Berge Lab at Erasmus MC, Netherlands
  
• Henner Farin Lab at Georg-Speyer-Haus, Institute for Tumorbiology and Experimental Therapy
• ​Dritan Agalliu's Lab at Columbia University Medical Center
  
• Robert Weinberg Lab at Whitehead Institute for Biomedical Research​​​​