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Recombinant Wnt Signaling Ligands
Wnt ligand stabilizer
Mouse Wnt Protein Control
Wnt Signaling Receptors
Wnt Ligand Stabilizer:
We proudly announce that we provide Wnt ligand stabilizer. the Stabilizer helps to maintain Wnt ligand activity for more than 30 hours in serum-free culture conditions.
Our Wnt ligands are intact, potent, and fully functional.
Potent: The Wnt3a has been proved three times more potent than any one else; 30 to 100 ng/mL for organoid formation;
Intact: These Wnt ligands are purified using a Wnt ligands-binding molecule-conjugated sepharose beads and size exclusion chromatography. The affinity chromatography yields a single pool with strong Wnt activity rather than two pools;
Fully functional: The size exclusion chromatography removes dimers and cleaved Wnt proteins. Compared to two Wnt peaks from affinity chromatography, the Wnt3a is fully functional.
We express and purify human Wnt3a from a special human cell line. Our human Wnt3a is as potent as the mouse one.
Ethan Lee lab at Vanderbilt -APC Inhibits Ligand-Independent Wnt Signaling by the Clathrin Endocytic Pathway. Developmental Cell 44, 566–581 (2018)
Leslie S. Gewin Lab at Vanderbilt -Blocking TGF-b and b-Catenin Epithelial Crosstalk Exacerbates CKD. Journal of the American Society of Nephrology 28; 3490-3503 (2017)
Lawrence Lum's Lab at UT Southwestern Medical Center - PNAS Early Edition, J. Moon
Organizations who have proved our Wnt ligands
The working concentrations of Wnt3a are