Recombinant Wnt Signaling Ligands



Product Name


Purity

Catalog


Wnt ligand stabilizer


N/A

bWps


Mouse Wnt Protein Control


N/A

rmWCtrl


Mouse Wnt3a​


Low Purity


rmW3aL


High Purity


rmW3aH

Human Wnt3a


Low Purity


rhW3aL

High Purity


rhW3aH
Human/Mouse Wnt5a

Low Purity

rhmW5aL

High Purity



Human Wnt5b

Low Purity


rhW5bL

High Purity



Human Wnt7b



Human Wnt10a



Human Wnt10b




Wnt Signaling Receptors


FZDs



LRPs



Copyright 2015. Time Bioscience. All Rights Reserved.

Recombinant Wnt Proteins


Wnt Ligand Stabilizer:


           We proudly announce that we provide Wnt ligand stabilizer. the Stabilizer helps to maintain Wnt ligand activity for more than 30 hours in serum-free culture conditions.


Wnt Ligands:


          Our Wnt ligands are intact, potent, and fully functional.


          Potent: The Wnt3a has been proved three times more potent than any one else; 30 to 100 ng/mL for organoid formation;


          Intact: These Wnt ligands are purified using a Wnt ligands-binding molecule-conjugated sepharose beads and size exclusion chromatography. The affinity chromatography yields a single pool with strong Wnt activity rather than two pools;


          Fully functional: The size exclusion chromatography removes dimers and cleaved Wnt proteins. Compared to two Wnt peaks from affinity chromatography, the Wnt3a is fully functional.


Human Wnt3a:


          We express and purify human Wnt3a from a special human cell line. Our human Wnt3a is as potent as the mouse one.


Publications:


Ethan Lee lab at Vanderbilt -APC Inhibits Ligand-Independent Wnt Signaling by the Clathrin Endocytic Pathway. Developmental Cell 44, 566–581 (2018)


Leslie S. Gewin Lab at Vanderbilt -Blocking TGF-b and b-Catenin Epithelial Crosstalk Exacerbates CKD. Journal of the American Society of Nephrology 28; 3490-3503 (2017)


Lawrence Lum's Lab at UT Southwestern Medical Center PNAS Early Edition, J. Moon


Organizations who have proved our Wnt ligands



The working concentrations of Wnt3a are

  • 1 to 10 ng/mL for our reporter cell lines (HEK293 or NIH3T3)
  • 10 to 20 ng/mL for MEF cell DVL2 phosphorylation
  • 50 to 100 ng/mL for organoid formation


Recombinant Stem Cell Growth Factors


Product Name

Purity
Catalog

Human Transforming Growth Factor

beta 1 (TGFβ1)



rhTGFB1


Human Noggin



rhNGN



Wnt Signaling Receptors


FZDs



LRPs