Copyright 2015. Time Bioscience. All Rights Reserved.


Recombinant Stem Cell Growth Factors


Product Name

Purity
Catalog

Human Transforming Growth Factor

beta 1 (TGFβ1)



rhTGFB1


Human Noggin



rhNGN



Wnt Signaling Receptors


FZDs



LRPs




Recombinant Wnt Signaling Ligands



Product Name


Purity

Catalog


Wnt ligand stabilizer


N/A

bWps


Mouse Wnt Protein Control


N/A

rmWCtrl


Mouse Wnt3a​


Low Purity


rmW3aL


High Purity


rmW3aH

Human Wnt3a


Low Purity


rhW3aL

High Purity


rhW3aH
Human/Mouse Wnt5a

Low Purity

rhmW5aL

High Purity



Human Wnt5b

Low Purity




High Purity



Human Wnt7b



Human Wnt10a



Human Wnt10b




Wnt Signaling Receptors


FZDs



LRPs



Recombinant Wnt Proteins


Wnt Ligand Stabilizer:


           We proudly announce that we provide Wnt ligand stabilizer. the Stabilizer helps to maintain Wnt ligand activity for more than 30 hours in serum-free culture conditions.


Wnt Ligands:


          Our Wnt ligands are intact, potent, and fully functional.


          Potent: The Wnt3a has been proved three times more potent than any one else; 30 to 100 ng/mL for organoid formation;


          Intact: These Wnt ligands are purified using a Wnt ligands-binding molecule-conjugated sepharose beads and size exclusion chromatography. The affinity chromatography yields a single pool with strong Wnt activity rather than two pools;


          Fully functional: The size exclusion chromatography removes dimers and cleaved Wnt proteins. Compared to two Wnt peaks from affinity chromatography, the Wnt3a is fully functional.


Human Wnt3a:


          We express and purify human Wnt3a from a special human cell line. Our human Wnt3a is as potent as the mouse one.


Publications:


Ethan Lee lab at Vanderbilt -APC Inhibits Ligand-Independent Wnt Signaling by the Clathrin Endocytic Pathway. Developmental Cell 44, 566–581 (2018)


Leslie S. Gewin Lab at Vanderbilt -Blocking TGF-b and b-Catenin Epithelial Crosstalk Exacerbates CKD. Journal of the American Society of Nephrology 28; 3490-3503 (2017)


Lawrence Lum's Lab at UT Southwestern Medical Center PNAS Early Edition, J. Moon


Organizations who have proved our Wnt ligands



The working concentrations of Wnt3a are

  • 1 to 10 ng/mL for our reporter cell lines (HEK293 or NIH3T3)
  • 10 to 20 ng/mL for MEF cell DVL2 phosphorylation
  • 50 to 100 ng/mL for organoid formation