Copyright 2015. Time Bioscience. All Rights Reserved.


We provide both highly potent Wnt ligands and their controls     


By credit card:


Please call: 617-319-2278


By PO:


Please send POto info@timebioscience.com

Recombinant Wnt Proteins

Our Wnt ligands are intact, potent, and fully functioned.


They have been evaluated by following organizations. These Wnt ligands are purified using a Wnt ligands-binding molecule-conjugated sepharose beads and size exclusion chromatography. The affinity chromatography yields a single peak with strong Wnt activity and the size exclusion removes dimers and cleaved Wnt proteins. Compared to two Wnt peaks from affinity chromatography, our Wnt3a is intact and fully functioned.


​We express and purify human Wnt3a from a special human cell line. Our human Wnt3a has been proved as potent as the mouse one.


Publications:


Leslie S. Gewin Lab at Vanderbilt - Journal of the American Society of Nephrology 28; 20017


Lawrence Lum's Lab at UT Southwestern Medical Center-PNAS Early Edition, J. Moon


The organizations who have proved our Wnt proteins


  • StemSynergy Therapeutics
  • Creative BioMart
  • Janssen  
  • Ethan Lee's Lab at Vanderbilt University School of Medicine
  • Xi He's Lab at Harvard Medical School
  • Dan Wu's Lab at Yale University School of Medicine
  • ​Anthony Brown's Lab at Weill Cornell Medicine
  • Henner Farin Lab, 30 ng/mL of Wnt3a support organoid formation
  • ​Dritan Agalliu's Lab, 40 ng/mL of Wnt3a works very well on purified primary brain endothelial cells
  • Robert Weinberg Lab,  beautiful gene changes of stem cell treated with Wnt3a

​​

The working concentrations of Wnt3a are

  • 1 to 10 ng/mL for our reporter cell lines (HEK293 or NIH3T3)
  • 10 to 20 ng/mL for MEF cell DVL2 phosphorylation
  • 30 to 100 ng/mL for organoid formation


Recombinant Wnt Signaling Ligands


Product Name

Purity

Catalog


Mouse Wnt Protein Control


N/A

rmWCtrl


Mouse Wnt3a


Low Purity


rmW3aL


High Purity


rmW3aH

Human Wnt3a


Low Purity


rhW3aL

High Purity


rhW3aH
Human/Mouse Wnt5a

Low Purity

rhmW5aL

High Purity



Human Wnt5b

Low Purity


rhW5bL

High Purity



Human Wnt7b



Human Wnt10a



Human Wnt10b




Wnt Signaling Receptors


FZDs



LRPs



How to order


Recombinant Stem Cell Growth Factors


Product Name

Purity
Catalog

Human Transforming Growth Factor

beta 1 (TGFβ1)



rhTGFB1


Human Noggin



rhNGN



Wnt Signaling Receptors


FZDs



LRPs